Identifikasi dan Karakterisasi Resistensi Antibiotik Terduga Staphylococcus aureus pada Susu Mastitis Subklinis asal Sapi Perah di Kelompok Ternak Sedyo Mulyo, Pakem, Sleman Yogyakarta.

Aziz, Fatkhanuddin; Lestari, Fajar B.; Indarjulianto, Soedarmanto; Fitriana, Fauziah

Staphylococcus aureus (S. aureus) is one of the bacteria that causing mastitis, implicate economic losses in dairy farm. Treatment of S. aureus is known to be difficult because of the mismatch of choosing the right type of antibiotic and the nature of resistance that is easily developed. The purpose of this study were to identified and characterized the antibiotic resistance of S. aureus in dairy cows subclinical mastitis from Sedyo Mulyo farm, Pakem, Sleman, Yogyakarta. Bacterial identification of S. aureus from 40 milk positive CMT test was carried out through bacterial culture with MSA test, coagulase, catalase, Gram staining, Voges-Proskauer test, and colony morphology on 5% sheep blood agar plates. Identification of S. aureus resistance to several antibtiotic were carried out by the Kirby-Bauer method. The results showed that 26 of 40 milk samples tested were presumptive identified for S. aureus. The percentage of antibiotics resistance to cefixim, ampicillin, oxytetracycline, penicillin G, erythromycin, and oxacillin, were 100%, 96%, 61.5%, 38.4%, 23%, and 2% respectively. Whereas all presumptive S. aureus isolates still 100% sensitive to ciprofloxacin. Monitoring and evaluation of the presence of S. aureus bacteria in subclinical mastitis milk needs to be done to prevent the potential for developing mastitis severity and antibiotic resistance.

Journal of Tropical Animal & Veterinary Sciences / Jurnal Ilmu Peternakan dan Veteriner Tropis, 2022, Vol 12, Issue 1, p66

2620-939X

Academic Journal

10.46549/jipvet.v12i1.226