In the present study we evaluated the chemical composition and allelopathic activity of the essential oil (EO) of Blepharocalyx salicifolius (Kunth) O. Berg leaves on the germination and initial growth of Lactuca sativa L. (dicotyledonous) and Sorghum bicolor (L.) Moench (monocotyledon). EO was obtained by hydrodistillation for 3 h. The EO composition analysis was performed by gas chromatography coupled to a mass spectrometry (GC-MS). The allelopathic activity of EO was evaluated at 2.5; 5.0 and 7.5%. The seeds were immersed in the solution for 2 min., distributed in Petri dishes, that were maintained in a BOD incubator. On the seventh day, the variables germination (%), root length (cm), shoot length (cm) and total seedling length (cm) were evaluated. The major constituents identified in the OE were eucalyptol (49.6%), limonene (13.6%) and β-caryophyllene (12.2%). The EO affected negatively the germination percentage of L. sativa seeds and the root system and total seedling length of S. bicolor. There was a negative allelopathic activity against the two evaluated species.