Malaricidal efficacy of the ethyl acetate fraction of Syzygium cumini fruit on spleen index, hemoglobin levels and mean survival time in mice (Mus musculus) infected with Plasmodium berghei.

Ilmayani, Arina; Maslachah, Lilik; Meles, Dewa Ketut; Setiawan, Boedi; Sugihartuti, Rahmi; Wahjuni, Retno Sri; Santoso, Kuncoro Puguh

Purpose: To determine the effectiveness of ethyl acetate fraction (EAF) of Syzygium cumini (Juwet fruit) on spleen index, hemoglobin levels and mean survival time of mice (Mus musculus) infected with Plasmodium berghei. Methods: A total of 60 mice were divided into 6 treatment groups of 10 mice each, namely N (not infected), K- (infected and untreated), K (infected, treated with 25 mg/kg chloroquine), P1 (infected, treated with 200 mg/kg ethyl acetate fraction), P2 (infected, treated with 400 mg/kg ethyl acetate fraction) and P3 (infected, treated with 600 mg/kg ethyl acetate fraction). The mice were each infected with 0.2 mL of 1 x 106 parasitized red blood cells (PRBCs) of P. berghei. Treatment was administered for 9 days after which spleen indices, hemoglobin levels and mean survival time were assessed. Results: The results showed significant difference in spleen index (p 0.05) was observed between K-, K , P1 and P3, as well as P2, K-, P1 and P3. The hemoglobin levels significantly differed (p 0.05) in the hemoglobin levels between P1, K , P2 and P3 as well as P2, K-, P1 and P3. Furthermore, there was a significant difference (p 0.05) between K-, P1, P2 and P3, as well as N, K , P1 and P2. Conclusion: The ethyl acetate fraction of S. cumini fruit neither reduce the spleen index nor increase the mean survival time of mice infected with P. berghei. However, the administration-maintains hemoglobin levels within the normal range of infected mice.

SURVIVAL rate; ERYTHROCYTES; ETHYL acetate; PLASMODIUM berghei; MICE

Tropical Journal of Pharmaceutical Research, 2024, Vol 23, Issue 11, p1857

1596-5996

Academic Journal

10.4314/tjpr.v23i11.8