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- Title
Pectolinarigenin - A Flavonoid Compound from Cirsium Japonicum with Potential Anti-proliferation Activity in MCF- 7 Breast Cancer Cell.
- Authors
Mingqian Lu; Qingzhi Kong; Xinhua Xu; Hongda Lu; Zhongxin Lu; Wei Yu; Bin Zuo; Jin Su; Rong Guo
- Abstract
Purpose: To isolate high purity pectolinarigenin from Cirsium japonicum and evaluates its antiproliferative potential and induction of apoptosis activity in MCF-7 breast cancer cell. Methods: Pectolinarigenin was purified by the combination of silica gel and Sephadex LH-20 column chromatography. The structure was identified by ¹H-nuclear magnetic resonance (NMR), 13C-NMR and electrospray ionization tandem mass spectrometry (ESI-MS) analyses. The anti-proliferation activity of pectolinarigenin was also evaluated by methylthiazolyldiphenyl-tetrazolium bromide (MTT) and colony formation assays in MCF-7 breast cancer cell. Results: Pectolinarigenin showed potent anti-proliferation activity by inducing apoptosis and downregulation of Bcl2 expression in MCF-7 breast cancer cell. Protein expression of Bcl2 was almost completely inhibited upon the treatment with 25 mM pectolinarigenin. Conclusion: A rapid method to isolate high purity pectolinarigenin from C. japonicum and as well as evaluate its anti-proliferative potential and induction of apoptosis in MCF-7 breast cancer cells is presented for the first time. Induction of apoptosis of MCF-7 is via downregulation of Bcl2 expression.
- Subjects
FLAVONOIDS; CIRSIUM; CELL proliferation; BREAST cancer treatment; APOPTOSIS; SILICA gel
- Publication
Tropical Journal of Pharmaceutical Research, 2014, Vol 13, Issue 2, p225
- ISSN
1596-5996
- Publication type
Academic Journal
- DOI
10.4314/tjpr.v13i2.9