Anti-Aging Effects of Hibiscus sabdariffa on Immortalized Human Ovarian Epithelial Cells.

Kılıç, Elif Dener; Parlak, Yüsra Nur; Yıldırım, Tuana; Çeşme, Merve; Akkaya, Selin; Amzım, Hafsa; Isanbaeva, Asalat; Özgültekin, Nurbanu; Farhangzad, Nazila; Gökmen, Derya; Özkan, Tülin; Bolkan, Aslıhan Gürbüz; Sunguroğlu, Asuman

ObjectivesThe aim of this study is to examine the anti-aging effect of Hibiscus sabdariffa (HS) against oxidative stress induced by hydrogen peroxide (H2O2) on immortalized human ovarian epithelial cells (IHOECs).Materials and MethodsThe obtained IHOECs were combined with 12.5 mg/mL HS extracted by adding 1mL of distilled water (dH2O) and different concentrations of H2O2 for oxidative effect. IHOECs cell viability was increased with HS extract and determined by MTT test assay. Real-time polymerase chain reaction was conducted to amplify and detect apoptosis-related BAX and BCL2, stem cell markers SOX2 and OCT4, and antioxidative nuclear factor erythroid 2-related factor 2 (NRF2) gene expressions. For the statistical analysis Kruskal-Wallis analysis and Mann-Whitney U test were used in SPSS program.ResultsThe increase in HS in cell proliferation was 14%. While there is no significant increase of cell viability in the combined group with HS and H2O2, there were only statistically significant groups are 200, 800, 900 and, 1,000 µl. It was indicated that HS has apoptotic, stem cell promotion and antioxidative effects on high H2O2 concentrations of these groups (p<0.05).ConclusionIn combined groups with both HS and H2O2 induced oxidative stress, HS increased the expression of proapoptotic gene BAX and decreased antiapoptotic gene BCL2. Although, the remaining cells which are abundantly stem cells expressed genes OCT4 and SOX2 remarkably decreased, at high H2O2 concentration opposite effects were observed. NRF2 gene expression also increased in this high oxidative stress.

NUCLEAR factor E2 related factor; ROSELLE; EPITHELIAL cells; STATISTICAL significance; OXIDATIVE stress; APOPTOSIS; STEM cells; MANN Whitney U Test; QUANTITATIVE research

Journal of Ankara University Faculty of Medicine / Ankara Üniversitesi Tip Fakültesi Mecmuasi, 2024, Vol 77, Issue 4, p374

0365-8104

Academic Journal

10.4274/atfm.galenos.2024.29053