Results
Title

Scopoletin from Cirsium setidens Increases Melanin Synthesis via CREB Phosphorylation in B16F10 Cells.

Authors

Mi-Ja Ahn; Sun-Jung Hur; Eun-Hyun Kim; Seung Hoon Lee; Jun Seob Shin; Myo-Kyoung Kim; Uchizono, James A.; Wan-Kyunn Whang; Dong-Seok Kim

Abstract

In this study, we isolated scopoletin from Cirsium setidens Nakai (Compositae) and tested its effects on melanogenesis. Scopoletin was not toxic to cells at concentrations less than 50 μM and increased melanin synthesis in a dose-dependent manner. As melanin synthesis increased, scopoletin stimulated the total tyrosinase activity, the rate-limiting enzyme of melanogenesis. In a cell-free system, however, scopoletin did not increase tyrosinase activity, indicating that scopoletin is not a direct activator of tyrosinase. Furthermore, Western blot analysis showed that scopoletin stimulated the production of microphthalmia-associated transcription factor (MITF) and tyrosinase expression via cAMP response element-binding protein (CREB) phosphorylation in a dose-dependent manner. Based on these results, preclinical and clinical studies are needed to assess the use of scopoletin for the treatment of vitiligo.

Subjects

SCOPOLETIN; CIRSIUM; MELANOGENESIS; MICROPHTHALMIA-associated transcription factor; CREB protein; PHOSPHORYLATION; MELANOMA; PHYSIOLOGY

Publication

Korean Journal of Physiology & Pharmacology, 2014, Vol 18, Issue 4, p307

ISSN

1226-4512

Publication type

Academic Journal

DOI

10.4196/kjpp.2014.18.4.307