Results
Title

Knockdown of ZBED6 is not associated with changes in murine preadipocyte proliferation or differentiation.

Authors

Zhang, Wei; Cline, Mark A; Liu, Dongmin; Gilbert, Elizabeth R

Abstract

ZBED6 was identified as a transcription factor that affects muscle mass and fat deposition in pigs. Mechanisms mediating effects on fat mass are unclear. The objective was to determine the effect of ZBED6 mRNA knockdown on 3T3-L1 preadipocyte differentiation and gene expression. Differentiation was associated with increased mRNA abundance of CEBP/α (P < 0.05), CEBP/β (P < 0.05), CEBP/δ (P < 0.05), FASN (P < 0.05), PPARγ (P < 0.05), and SREBP-1 (P < 0.05), and decreased abundance of PREF-1 (P < 0.05). Knockdown of ZBED6 was not associated with changes in mRNA abundance of selected genes, lipid accumulation, lipid droplet size, or cell number. These results suggest that ZBED6 does not play a major role in preadipocyte differentiation.

Subjects

FAT cells; CELL differentiation; CELL proliferation; TRANSCRIPTION factors; GENE expression; MESSENGER RNA; ADIPOGENESIS

Publication

Adipocyte, 2013, Vol 2, Issue 4, p251

ISSN

2162-3945

Publication type

Academic Journal

DOI

10.4161/adip.26028