Neuroprotective effects of coffee-derived exosome-like nanoparticles against Aβ-induced neurotoxicity.

Esmekaya, Meric A.; Ertekin, Burhan

The present study aimed to provide experimental evidence that CDELNs (coffee-derived exosome-like nanoparticles) may be a candidate for the treatment or prevention of amyloid-ß (Aß)- induced Alzheimer's disease (AD). An in vitro Alzheimer's model was created with Aß-induced toxicity in mouse hippocampal neuronal cells (HT-22). Aß(1-42)-exposed cells were treated with different concentrations of CDELNs (1-50 µg/ml) and the viability of cells was analyzed. The change in the mitochondrial membrane potential (∆Ψm) of cells was also determined. CDELNs treatment increased the viability of Aß(1-42)-toxicity-induced HT-22 cells significantly. The increase in the viability of Aß(1-42)-toxicity-induced cells was correlated with an improvement in ∆Ψm. CDELNs treatment restored the dissipated ∆Ψm. These results suggested that CDELNs protect neuronal cells against Aß(1-42)-induced neurotoxicity by repairing mitochondrial dysfunction. CDELNs might be a useful neuroprotective agent for the treatment or prevention of Aß-induced AD. Further animal and clinical studies should be carried out to investigate the neuroprotective potential of CDELNs against Aß-induced AD.

ALZHEIMER'S disease; MEMBRANE potential; MITOCHONDRIAL membranes; CELL survival; NEUROPROTECTIVE agents

General Physiology & Biophysics, 2024, Vol 43, Issue 6, p535

0231-5882

Academic Journal

10.4149/gpb_2024025