Results
Title

Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts.

Authors

Yang, Shun-Kai; Leo, Teik-Kee; Mai, Chun-Wai; Chin, Swee-Yee; Lamasudin, Dhilia; Lim, Swee-Hua-Erin; Tan, Ngai-Paing; Lai, Kok-Song

Abstract

Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process.

Subjects

ASIA; REACTIVE oxygen species; PROTEIN expression; SODIUM dodecyl sulfate; GEL electrophoresis; EXTRACTS

Publication

Pharmacognosy Magazine, 2020, Vol 16, Issue 70, p431

ISSN

0973-1296

Publication type

Academic Journal

DOI

10.4103/pm.pm_281_19