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- Title
Protein expression patterns in hek-Blue™ - Cells treated with Clinacanthus nutans extracts.
- Authors
Yang, Shun-Kai; Leo, Teik-Kee; Mai, Chun-Wai; Chin, Swee-Yee; Lamasudin, Dhilia; Lim, Swee-Hua-Erin; Tan, Ngai-Paing; Lai, Kok-Song
- Abstract
Background: Clinacanthus nutans (CN) is a small shrub native to tropical Asia known for their anti-oxidant, anti-inflammation, anti-cancer, and anti-viral activities. Objectives: This study aimed to investigate the effect of CN extract on human embryonic kidney cell line (HEK-Blue™-4) in a proteomic perspective. Materials and Methods: Comparative proteomic profiling through two-dimensional sodium dodecyl sulfate gel electrophoresis was performed on HEK-Blue™-4 treated with CN leaf polar extract. Results: We successfully identified seven upregulated proteins, of which five promoted the growth of the HEK-Blue™-4 cells. Interestingly, a potent antioxidant enzyme which neutralizes reactive oxygen or nitrogen species, peroxiredoxin-1 was also upregulated in HEK-Blue™-4 cell lines after treatment with CN leaf polar extract. Conclusion: CN leaf polar extract promotes the growth of HEK-Blue™-4 cells and induced the expression of peroxiredoxin-1, which protects the cells from reactive oxygen species during the inflammation process.
- Subjects
ASIA; REACTIVE oxygen species; PROTEIN expression; SODIUM dodecyl sulfate; GEL electrophoresis; EXTRACTS
- Publication
Pharmacognosy Magazine, 2020, Vol 16, Issue 70, p431
- ISSN
0973-1296
- Publication type
Academic Journal
- DOI
10.4103/pm.pm_281_19