Results
Title

Lack of human papillomavirus DNA in colon adenocarcinama and adenoma.

Authors

Aghakhani, Arezoo; Hamkar, Rasool; Ramezani, Amitis; Bidari-Zerehpoosh, Farahnaz; Sabeti, Shahram; Ghavami, Nastaran; Banifazl, Mohammad; Rashidi, Niloofar; Eslamifar, Ali

Abstract

Background: Human papilloma viruses (HPV) have been detected in several types of cancers. Over the last few years, a possible correlation between HPV infection and colon cancers has been suggested. The aim of this study was to assess the presence of HPV-DNA in colon adenocarcinomas and adenomas to describe the relationship of HPV infection with these pathologic features. Materials and Methods: The study included formalin-fixed and paraffin-embedded tissue samples of 70 patients with colon adenocarcinoma and 30 patients with adenoma (as study group) and 30 tumor adjacent tissues (as control). HPV-DNA was purified and first amplified through MY09/MY11 and GP5 /GP6 primers. Subsequently, for more precision, another PCR was performed using PGMY09/11 L1 consensus primer system. Results: All tested adenocarcinomas and adenomas as well as normal tumor adjacent tissues were negative for all types of HPV in two PCR assays. Conclusion: Our results do not support the relationship between HPV infection and colon carcinoma or adenoma. Attributing a role to the HPV in the etiology of colon carcinogenesis will require further studies.

Subjects

COLON cancer; PAPILLOMAVIRUSES; DNA viruses; ADENOMA; ADENOCARCINOMA; ETIOLOGY of cancer

Publication

Journal of Cancer Research & Therapeutics, 2014, Vol 10, Issue 3, p531

ISSN

0973-1482

Publication type

Academic Journal

DOI

10.4103/0973-1482.137674