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- Title
Cellular responses to 3D printed dental resins produced using a manufacturer recommended printer versus a third party printer.
- Authors
Sona Cardoso, Beatriz; Brito da Cruz, Mariana; Faria Marques, Joana; Carlos Roque, João; Paulo Martins, João; Cordeiro Malheiro, Rodrigo; Duarte da Mata, António
- Abstract
PURPOSE. The aim of this study was to evaluate the influence of different 3D dental resins, using a manufacturer recommended printer and a thirdparty printer, on cellular responses of human gingival cells. MATERIALS AND METHODS. Three NextDent resins (Denture 3D , C&B MFH and Crowntec) were used to produce specimens on printers NextDent 5100 (groups ND, NC and NT, respectively) and Phrozen Sonic Mini 4K (groups PD, PC and PT, respectively). Human gingival fibroblasts were cultured and biocompatibility was evaluated on days 1, 3 and 7. IL-6 and IL-8 concentrations were evaluated at 3 days using ELISA. Surface roughness was evaluated by a contact profilometer. SEM and fluorescence micrographs were analyzed at days 1 and 7. Statistical analyses were performed using SPSS and mean differences were tested using ANOVA and posthoc Tukey tests (P < .05). RESULTS. There was an increase in cellular viability after 7 days in groups PC and PT, when compared to group PD. ND group resulted in higher concentration of IL-6 when compared to PT group. SEM and fluorescence micrographs showed less adhesion and thinner morphology of fibroblasts from group PD. No significant differences were found regarding surface roughness. CONCLUSION. The use of different printers or resins did not seem to influence surface roughness. NextDent 5100 and Phrozen Sonic Mini 4K produced resins with similar cellular responses in human gingival fibroblasts. However, Denture 3D resin resulted in significantly lower biocompatibility, when compared to C&B MFH and Crowntec resins. Further testing is required to support its long-term use, required for complete dentures.
- Subjects
DENTAL resins; COMPLETE dentures; MANUFACTURING industries; SURFACE roughness; FIBROBLASTS
- Publication
Journal of Advanced Prosthodontics, 2024, Vol 16, Issue 2, p126
- ISSN
2005-7806
- Publication type
Academic Journal
- DOI
10.4047/jap.2024.16.2.126