EX VIVO ANALYSIS OF PACKED RED BLOOD CELL HEMOLYSIS DURING TRANSFUSION BY PNEUMATIC PRESSURE BAGS.

da Luz Gonçalves Pedreira, Mavilde; de Oliveira Pires, Maria Paula; Belela-Anacleto, Aline Santa Cruz; Myiuki Kusahara, Denise; Affonso Fonseca, Fernando Luiz; Perez Pardo, Larissa; Sorgini Peterlini, Maria Angélica

Objective: to investigate markers of red cell hemolysis in stored blood infused by pneumatic pressure bags under a pressure of 300 mmHg. Methods: ex vivo experimental study simulating the clinical practice of massive packed red blood cell transfusion by pressure bags. The experiments were carried out under controlled conditions in a laboratory and the levels of hemolysis markers [total hemoglobin (g/dL), hematocrit (%), free hemoglobin (g/dL), potassium (mmol/L), lactate dehydrogenase (U/L) and degree of hemolysis (%)] were assessed before device inflation (control), and immediately, one and two hours after inflation under a pressure of 300 mmHg. Data were analyzed with parametric and non-parametric tests and the significance level was set at 5%. Results: the analysis showed increased free hemoglobin level (p=0.004) and hemolysis ratio (p=0.004) immediately after compression, increased lactate dehydrogenase one hour after compression (p=0.020), and decreased total hemoglobin (p=0.002) two hours after compression, without significant variations in potassium or hematocrit levels. Conclusion: the use of pressure bags for infusion of red blood cells caused alterations in hemolysis markers mainly after two hours of compression.

RED blood cell transfusion; STATISTICAL models; ERYTHROCYTES; PATIENT safety; T-test (Statistics); DATA analysis; RESEARCH funding; HEMOGLOBINS; POTASSIUM; KRUSKAL-Wallis Test; LACTATE dehydrogenase; DESCRIPTIVE statistics; MANN Whitney U Test; EXPERIMENTAL design; HEMATOCRIT; INFERENTIAL statistics; FRIEDMAN test (Statistics); ANALYSIS of variance; STATISTICS; HEMOLYSIS & hemolysins; DATA analysis software; NONPARAMETRIC statistics

Ciencia, Cuidado e Saude, 2024, Vol 23, p1

1677-3861

Academic Journal

10.4025/ciencuidsaude.v23i0.66134