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- Title
高效液相色谱-蒸发光散射法测定脂质体中神经酰胺NP 和AP含量.
- Authors
赵格格; 苏温柔; 王飞飞
- Abstract
Simultaneous chromatographic separations of ceramide NP and ceramide AP in ceramide liposomes were carried out by high-performance liquid chromatography( HPLC) coupled to evaporative light scattering detector( ELSD). The ceramidelipo somes samples were treated with methanol-isopropanol( volume ratio 7∶3) and analyzed by Agilent ZORBAX StableBond C8( 4.6×150 mm, 3.5 μm) column. The simultaneous separation of ceramide NP and AP was achieved by gradient elution with 0.1% formic acid in methanol-isopropanol( volume ratio 7∶3) and acetonitrile as the mobile phases. The flow rate was 1.0 mL/min and the column temperature was 40 ℃. Evaporator and nebulizer temperature were set at 90 ℃ and 70 ℃, and the gas flow rate was 1.0 L/min. The result shows the detection limits of ceramide NP and AP in ceramide liposomes are 3 and 6 μg/mL, respectively, and the quantitative limits are 1 and 2 μg/mL, respectively. The RSD of test on ceramide NP and AP repeatability and precision are all less than 2%, and the linear relationship of calibration curves for ceramide NP and AP is good( R2>0.999) in the mass concentration range of 50-500 μg/mL and 25-250 μg/mL, respectively. Moreover, the recovery rates range from 96.2% to 105.1% at different concentration levels. A simple, reproducible and reliable method is provided for the determination of ceramide NP and AP contents in different ceramide liposomes.
- Subjects
HIGH performance liquid chromatography; PHOTODETECTORS; LIGHT scattering; CERAMIDES; LIPOSOMES
- Publication
China Surfactant Detergent & Cosmetics (2097-2806), 2023, Vol 53, Issue 12, p1466
- ISSN
2097-2806
- Publication type
Academic Journal
- DOI
10.3969/j.issn.2097-2806.2023.12.015