We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
不同耐药型的结核分枝杆菌hspX基因序列与表达的比较.
- Authors
杨瑜; 王楠; 李华; 吴玲; 王琪; 谢贝; 刘志辉; 孟繁荣
- Abstract
Objective To preliminarily explore whether there is a correlation between heat shock protein gene hspX and drug resistance of Mycobacterium tuberculosis (MTB) by comparing the sequence and expression of hspX in MTB with cliff erent drug resistance types. Methods Ten strains sensitive to all four first-line anti-tuberculosis drugs (S), one drug resistance (rifampicinresistant MTBR+, isoniazid-resistant MTBH+, streptomycin-resistant MTBS+ and ethambutol-resistant MTBE+) and multidrug resistance (MDR) to isoniazid and rifampicin were selected, respectively. The DNA and RNA of strains in each group were extracted by modified SDS lysis method and TRlzol method, respectively. The existence and expression of hspX gene were determined by PCR and RTPCR using specific primers. PCR products were sequenced to check the forward and reverse polymorphism, and DNAman software was employed to compare the sequence with the standard strain H37Rv. The cliff erence in the expression of hspX among MTB strains with different drug resistance types was analyzed by pairwise with the sensitive strain group as the control group. Results The specific hspX gene was successfully obtained by PCR. The hspX gene sequence was 97%-99% identical with H37Rv, and there was no significant cliff erence. The mean expression level of hspX through relative quantitation i-'"' in S, MDR, MTBR+, MTBH+, MTBS+ and MTBE+ strains was calculated as 0.98 (0.89, 1.23), 1.49 (1.10, 2.04), 1.41 (0.55, 2.80), 1.37 (0.68, 2.71), 0.91 (0.59, 1.62) and 0.70 (0.48, 1.18), respectively. There was no significant cliff erence in the expression of hspX among cliff erent drug-resistant strains groups when the sensitive strain group was regarded as the control group (all P > 0.05) . Conclusion The hspX gene is conserved in MTB. In natural state, there is no significant correlation between hspX gene and drug resistance of MTB.
- Subjects
GENE expression; HEAT shock proteins; ANTITUBERCULAR agents; MULTIDRUG resistance; DRUG resistance
- Publication
Journal of New Medicine, 2023, Vol 54, Issue 5, p340
- ISSN
0253-9802
- Publication type
Academic Journal
- DOI
10.3969/j.issn.0253-9802.2023.05.007