Purple sweet potato anthocyanin regulates the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through circ_0003998/miR-145 axis.

MA Jianping; SONG Lianchuan; ZHAO Chengmao; LYU Yong; LI Hua; WANG Xuechang

Objective: To investigate whether purple sweet potato anthocyanin (PSPA) regulates the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through the circ&lowbar;0003998/miRNA-145 axis. Methods: Breast cancer MDA-MB-231 cells were divided into control group, PSPA groups (200, 400, 800 μg/ml PSPA), pcDNA group, pcDNA-circ&lowbar;0003998 group, si-NC group, si-circ&lowbar;0003998 group, si-circ&lowbar;0003998 anti-miR-145 group, PSPA pcDNA group, PSPA pcDNA-circ&lowbar;0003998 group and PSPA anti-miR-145 group. The expressions of circ&lowbar;0003998 and miR-145 in MDA-MB-231 cells were detected by qPCR method. The cell proliferation, migration and invasion abilities were measured by CCK-8 method and Transwell chamber method, respectively. WB was used to detect the protein expressions of Ki-67, MMP-2 and MMP-9 in cells. Dual luciferase reporter gene assay was used to analyze the targeting relationship between circ&lowbar;0003998 and miR-145. Results: Compared with the control group, the proliferation inhibition rate and miR-145 expression level in MDA-MB-231 cells of various PSPA groups increased significantly, while the expression of circ&lowbar;0003998 and protein expression levels of Ki-67, MMP-2, MMP-9, as well as the number of migrated and invaded cells were significantly reduced (all P<0.01), all of which were in a concentration-dependent manner. circ&lowbar;0003998 could target and negatively regulate the expression of miR-145. After inhibiting circ&lowbar;0003998, the proliferation inhibition rate and the expression level of miR-145 in MDA-MB-231 cells were significantly increased, while the protein expression levels of Ki-67, MMP-2 and MMP-9, and the number of migrated and invaded cells were significantly reduced (all P<0.01). After co-transfection of si-circ&lowbar;0003998 and anti-miR-145, the suppression effect of circ&lowbar;0003998 inhibition on the proliferation, migration and invasion of MDA-MB-231 cells were reversed. circ&lowbar;0003998 overexpression or miR-145 inhibition could reverse the inhibitory effects of PSPA on the proliferation, migration and invasion of MDA-MB-231 cells. Conclusion: PSPA inhibites the proliferation, migration and invasion of breast cancer MDA-MB-231 cells through the circ&lowbar;0003998/miR-145 axis.

Chinese Journal of Cancer Biotherapy, 2021, Vol 28, Issue 7, p672

1007-385X

Academic Journal

10.3872/j.issn.1007-385x.2021.07.004