Aim: The triple screening test is used to detect certain fetal chromosomal abnormalities, especially Down syndrome. In addition, studies have found that it is also associated with adverse pregnancy outcomes. In our study, we aimed to determine whether there is a relationship between serum free estriol levels, one of the triple test parameters, and the blood pH values of the babies of patients who underwent cesarean section due to fetal distress. Materials and Methods: A total of 901 pregnant women who underwent cesarean section due to fetal distress during labor at Ankara Bilkent City Hospital's Department of Obstetrics and Gynecology between January 1, 2022, and December 31, 2022, were included in the study. A total of 270 pregnant women who underwent second trimester screening at our hospital were included in the study. Maternal age, body mass index (BMI), gestational age, labor process and triple test results, along with each newborn's postnatal umbilical artery blood gas values, were recorded. Results: Patients were divided into two groups based on their estriol values: below 1 mom (group 1, n=163) and above 1 mom (group 2, n=107). There were no statistically significant differences between the groups in terms of maternal age, BMI, gestational age, induction of labor, APGAR score, presence of meconium, newborn sex, and neonatal intensive care unit admission rates (p>0.05). The umbilical cord pH value was determined to be 7.26 ± 0.51 in group 1 and 7.32 ± 0.08 in group 2 (p: 0.188). Conclusion: Although not statistically significant, the umbilical artery pH level in newborns of mothers who underwent cesarean section due to fetal distress is lower in those with second trimester serum free estriol levels below 1.