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- Title
lncRNAs as New Biomarkers in Systemic Lupus Erythematosus: A Prospective Study.
- Authors
Shaker, Olfat Gamil; Nassar, Yasser Hussein; Gheta, Tamer Atef; Essameldin Moussa Erfan, Randa Mohamed
- Abstract
Aim: To investigate serum levels of two Immune-related functional lncRNAs, growth arrest-specific transcript 5 (GAS5) and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), in Egyptian patients with SLE and to evaluate their relationship with disease activity. Method: The present study was a case-control study that was carried out on 39 patients with SLE and 46 age and sex-matched healthy controls. The expression levels of GAS5 and MALAT1 were measured using by real-time polymerase chain reaction (PCR). Results: There were statistically significant differences between cases and controls in GAS5 (p <0.001) and MALAT1 expression (p <0.01). The mean GAS5 was significantly higher in the control; while the mean MALATI expression was significantly higher in SLE patients. The ROC curve revealed that GAS5 was a good discriminant with AUC 0.849 with sensitivity 93.5% and specificity 74.3%. Moreover, MALAT1 was a good discriminant to differentiate cases from controls with AUC 0.3 with 95% CI (0.162 - 0.438), the most suitable cut-off point was ≥ 2.1 with Sensitivity 93.5% and Specificity 72.5%. Conclusion: GAS5 and MALAT1 may serve as potential biomarkers for the diagnosis and monitoring of the SLE, both lnRNAs exhibited a good diagnostic accuracy to discriminate between SLE patients and healthy controls.
- Subjects
SYSTEMIC lupus erythematosus; LONGITUDINAL method; POLYMERASE chain reaction; BIOMARKERS; RECEIVER operating characteristic curves
- Publication
Indian Journal of Public Health Research & Development, 2020, Vol 11, Issue 2, p2509
- ISSN
0976-0245
- Publication type
Academic Journal
- DOI
10.37506/v11/i2/2020/ijphrd/195238