A Real-Time Polymerase Chain Reaction assay for the Simultaneous Detection of Sexually Transmitted Pathogens in Women.

Taher, Noor M.; Salman, Haleema

Genital tract infection is a major source of morbidity in sexually active individuals. Reported rates of STD underestimate the exact problem of infection because the majority of STDs are asymptomatic and therefore go undetected especially in our community. The aim of this study was to investigate for the prevalence STD pathogens in females. 184 urogenital swabs collected from outpatient females then analyzed by Real-Time PCR for eight different pathogens (Neisseria gonorrhoeae, Chlamydia trachomatis, Herpes simplex virus, Trichomonas vaginalis, Hepatitis viruses B & C, Mycoplasma genitalium and Human papillomavirus).90.21% of samples resulted positive for one of the targets named above. PCR is a beneficial diagnostic technique for STDs since it has a highly specific and sensitive. the most prevalence pathogen was Human papilloma virus 40.96%.

POLYMERASE chain reaction; GENITALIA infections; PATHOGENIC microorganisms; PAPILLOMAVIRUSES; NEISSERIA gonorrhoeae

Indian Journal of Public Health Research & Development, 2020, Vol 11, Issue 2, p2577

0976-0245

Academic Journal

10.37506/v11/i2/2020/ijphrd/195218