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- Title
Improvement in Vitro Fertilization and Embryo Cleavage Rate of Mice Oocytes by Using Repaglinide with Supplementation of IVF Medium.
- Authors
Awadh, Sura A.; Azadbakht, Mehri; Alhady, Faris N. A.
- Abstract
Considering to progresses in the scientific reproductive research in both clinical medicine and basic science, in vitro fertilization (IVF) is one of the assisted reproductive technologies (ART) that is widely used for treatment infertility and increasing of pregnancy rate at infertile couples. In this study it was examined the effect of repaglinide on in vitro fertilization and embryo cleavage in mice oocytes. It was used female NMRI mice at 8 weeks old that after superovulation with injecting 5 IU of PMSG, followed by injection 5 IU of hCG at 48 h later. After preparation of sperm for in vitro insemination, MII oocytes randomly divided in two groups; control without repaglinide in IVF medium and treatment with 1μM concentration of repaglinide in IVF medium. Fertilized 4–6 h post IVF were oocytes washed and put in 50 μl droplets of culture medium. IVF and embryo development was assessed for 24, 48, 72, 96 and 120 h. The results showed that, in vitro fertilization, embryonic cleavage rate and the parameters of blastocysts quality such as the total cell number, trophectoderm (TE), ratio of TE/ICM and size of blastocyst in the repaglinide group were significantly higher than the control.
- Subjects
FERTILIZATION in vitro; CLEAVAGE (Embryology); EMBRYOS; REPRODUCTIVE technology; CLINICAL medicine research
- Publication
Indian Journal of Public Health Research & Development, 2020, Vol 11, Issue 2, p2463
- ISSN
0976-0245
- Publication type
Academic Journal
- DOI
10.37506/v11/i2/2020/ijphrd/195207