Results
Title

Molecular Detection of Human Cytomegalovirus and Immunohistochemical Expression of BCL2 in Lymph Nodes from Hodgkin’s Lymphoma.

Authors

Al-Rubaie, Haitham Ahmed; Mohammed Ali, Saad Hasan; Hussein, Mustafa Jaffar; Mohammed Al-Alwany, Shakir H.

Abstract

Background: A lifelong persistence of latent human cytomegalovirus (hCMV) infection may result and/or associated with Hodgkin’s lymphoma (HL). The B-cell lymphoma-2 (BCL2) is an anti-apoptotic molecule expressed in non-neoplastic lymphoid tissue (hyperplastic lymph node) while down-regulated in normal germinal center B cells. Objective: Is to analyze the concordant impact of BCL2 expression and hCMV infection on tissues from a group of patients with Hodgkin’s lymphoma. Patients and Method: Eighty formalin-fixed, paraffin-embedded lymph node tissue-biopsies enrolled in this study; 40 from HLand 40 with unremarkable pathological changes. Detection of hCMV was done by chromogenic in situ hybridization (CISH) technique while immunohistochemistry (IHC) was used for the BCL2 protein expression. Results: In HL, the hCMV-positiveCISH and the BCL2-positive IHC reactions were detected in 35% and 42.5% of tissues, respectively. The correlation between hCMV and BCL2 was highly significant (P= 0.002). Conclusions: The significant co-expression of BCL2 and hCMV genes in HL tissues could point for their possible role in either lymph node pathogenesis or carcinogenesis.

Subjects

HODGKIN'S disease; LYMPH nodes; HUMAN cytomegalovirus; LYMPHOID tissue; PATHOLOGY

Publication

Indian Journal of Public Health Research & Development, 2020, Vol 11, Issue 2, p2380

ISSN

0976-0245

Publication type

Academic Journal

DOI

10.37506/v11/i2/2020/ijphrd/195193