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- Title
Curcumin and 6-Shogaol Increase Hemoglobin F Levels by Inhibiting Expression of STAT3 mRNA Gene in K562 Line Cell.
- Authors
Setyono, Joko; Sadewa, Ahmad Hamim; Meiyanto, Edy; Mustofa, Mustofa.
- Abstract
One of the approaches for beta-thalassemia therapy is the induction of the Haemoglobin F (Hb F). Curcumin and 6-Shogaol are empirically known to induce HbF, but the signalling cascade has not been widely explained. This study aims to uncover the potential of Curcumin and 6-shogaol in inhibiting the expression of STAT3 mRNA gene.This study uses the K562 erythroleukemic line cell model with an experimental design post-test only with a control group. There are 5 groups, each group has 3 replications, named the control group without treatment, the positive control group with Hydroxyurea treatment (75 μM), the combination treatment group of Curcumin (2 μM) and 6-Shogaol (10 μM), the single curcumin (2 μM) treatment group and a single 6-Shogaol (10 μM) treatment group. Test samples were taken in 72-h and 96-h time series, then RNA extraction from the cell line was continued by cDNA synthesis. The expression of STAT3 mRNA gene was measured using the qRT-PCR technique; then, the Hb F level was measured by the ELISA method. Statistical analysis using ANOVA test with significance level p 0.05) compared to the control group, but in the positive control group (p> 0.05) and the combination group (p> 0.05) it is lower than the control group without any treatment. Curcumin and 6-shogaol increase Hb F levels through inhibition expression of STAT3 mRNA Gene on K562 cells. The results of this study could be the basis for further research in vivo to reveal the signalling pathway in Hb Finduction therapy.
- Subjects
CURCUMIN; CELL lines; MESSENGER RNA; HEMOGLOBINS; STATISTICAL hypothesis testing; FETAL hemoglobin
- Publication
Indian Journal of Public Health Research & Development, 2020, Vol 11, Issue 2, p1263
- ISSN
0976-0245
- Publication type
Academic Journal
- DOI
10.37506/v11/i2/2020/ijphrd/194994