Forensic Assessment of Genotoxic Effects of Lead Metal on leaf Phragmites australis Using Random Amplified Polymorphic DNA (RAPD-PCR) Markers.

Ibraheem, Ahmed Ghalib; Ajmi, Reyam Naji

The influence lead metal on DNA integrity in leaf plant can measure by potential effects of pollutants accumulation metal, Pb in leaf plant Phragmites australis was subjected to RAPD analysis, have been found metal at concentrations of S1, S2,S3 (0.045, 2.97, 0.93) Respectively. polymorphisms became evident as the presence and absence of DNA fragments in affected samples compared to effect samples. Five prime was selected OP-L05, OP-M14, OP-M20, OP-V09 and OP-M05 were used, the genomic DNA of leaf did not appear polymorphic band at OP-L05 except molecular weight 600bp and presence of polymorphic band, OP-M20 show only polymorphic band at 850bp, OP-M05 did not show any polymorphisms and OP-M14, OP-V09 primers appeared polymorphisms when compare unexposed in control, this study confirmed RAPD markers a fast and simple technique can be used detection of forensic environmental.

METAL foils; PHRAGMITES australis; PHRAGMITES; DNA; ENVIRONMENTAL forensics; MOLECULAR weights; FOLIAGE plants

Indian Journal of Forensic Medicine & Toxicology, 2021, Vol 15, Issue 3, p1515

0973-9122

Academic Journal

10.37506/ijfmt.v15i3.15520