Background: Tobacco use is a critical global health issue, with higher prevalence among women in India’s tribal communities. This study evaluates a tobacco cessation intervention for reproductive-aged women in the Akole Tribal Block, Ahmednagar district, Maharashtra, India. Materials and Methods: A quasi-experimental design with 212 participants was employed using multi – stage sampling: cluster random sampling followed by simple random sampling. The study involved pre-test and post-test phases with data collected via a structured questionnaire. The intervention utilized the 5A model for tobacco cessation, with follow-up after 30 days. Data analysis was conducted using R software, with paired t-tests for score comparisons and multiple linear regression to assess socio-demographic influences on post-test scores. Results: Knowledge about tobacco-related diseases improved significantly, with mean scores rising post-intervention. Smokeless tobacco consumption decreased from 5.45 to 5.19 units/day t(167) = 3.55, p < 0.01), although the duration of tobacco use remained unchanged. The Fagerstrom Nicotine Dependence Scale scores slightly improved from 7.40 to 7.19 with t (167) = 6.01, p < 0.01). Education was marginally significant, and marital status was the only significant predictor for post-test dependence scores. Conclusion: The intervention demonstrated promise in increasing knowledge and slightly reducing tobacco consumption. However, a more culturally tailored and comprehensive strategy is needed, combining behavioural therapy with pharmacological support, to effectively address the entrenched habit of smokeless tobacco use among these women.