Results
Title

Formulation and evaluation of an ointment from Pinus gerardiana extracts indigenous to Balochistan.

Authors

Shahwani, Ghulam Mustafa; Jan, Syed Umer; Akhtar, Muhammad; Razzaque, Ghulam; Gul, Rahman; Ul Haq, Noman; Arsalan, Muhammad; Wahid, Abdul; Farooq, Ghulam; Qadir, Abdul

Abstract

In skin disorders such as microbial and fungal infections, plants and their parts are used. However, there have been very few scientific reports of herbal extracts of the plant Pinus gerardiana to be administered transdermally. The antifungal activity was assessed using poisoned food method against the strains of three pathogenic fungi, namely Alternaria alternata, Curvularia lunata and Bipolaris specifera. Ointment was prepared according to British pharmacopeia and physiochemical evaluation tests were performed. The GCMS was used to determine the chemical composition of the essential oil of Pinus gerardiana. 27 components were obtained. Monoterpenes= 89.97%, Oxygenated monoterpenes = 8.75%, Sesquiterpenes = 2.21% out of 100% of the total composition. The extract of pinus gerardiana showed a zone of inhibition on organism Bipolaris specifera 2.98±0.1µg/ml, Alternaria alternate 3.48±0.21µ/ml and Curvularia lunata 5.04±0.24µg/ml. Ointment was prepared with pH 5.9, conductivity 0.1, viscosity 22.24 and tested for stability. Franz cells were used in vitro, and release was determined from 30 minutes to 12 hours.

Subjects

PINE; ALTERNARIA alternata; PATHOGENIC fungi; ESSENTIAL oils; ALTERNARIA

Publication

Pakistan Journal of Pharmaceutical Sciences, 2022, Vol 35, p1819

ISSN

1011-601X

Publication type

Academic Journal

DOI

10.36721/PJPS.2022.35.6.SP.1819-1825.1