Anti-inflammatory potential of spectroscopically analyzed trans-13-octadecenoic acid of Yucca elephantipes Regel roots: in-vitro and in-vivo analysis.

Khan, Muhammad Shoaib; Uzair, Muhammad; Hanif, Muhammad; Jabeen, Mehreen; Chaudhary, Bashir Ahmad

Plants are always better choice for treatment of disease in contrast to synthetic agents due to their less toxicity, and free availability with minor limitations of identifications, purity, and potency which should be addressed. The objective of current study was to explore the anti-inflammatory effect of methanol extract of Yucca elephantipes roots by using oxidative burst assay and carrageen an induced rat paw edema. GC-MS analysis was carried for the determination of ani-inflammatory potential of fatty acids and other phytochemicals present in Yucca elephantipes roots. Among fifteen detected compounds trans-13-octadecenoic acid, n-hexadecanoic acid and 4-hydroxy benzoic acid were found as 84.21%, 5.21% and 2.17% respectively. Oxidative burst assay showed anti-inflammatory potential of Yucca elephantipes roots 74.58±0.32% with IC50 of 15.3±2.2μg/mL as compared to Ibuprofen with percentage of inhibition 73.20±0.17% and IC50 was 11.2±0.98μg/mL. Fortunately, less than 8g/kg dose of the Yucca elephantipes roots found safe in albino rats. Interestingly, in-vivo carrageen an induced paw edema method proved its anti-inflammatory potential at dose 100 and 200 mg/kg in albino rats. Conclusively, 200 mg/kg dose of Yucca elephantipes roots extract was optimized for 88.89±0.015 % anti-inflammatory effect which can be considered most potent, safe and better alternative of synthetic drugs.

BENZOIC acid; THERAPEUTICS; CONTRAST media; SYNTHETIC drugs; ANTIARTHRITIC agents; ACIDS; HYDROXYCINNAMIC acids

Pakistan Journal of Pharmaceutical Sciences, 2022, Vol 35, Issue 6, p1549

1011-601X

Academic Journal

10.36721/PJPS.2022.35.6.REG.1549-1556.1