INTRACAMERAL INJECTION OF LIMBAL MESENCHYMAL STEM CELLS CONDITIONED MEDIA (LMSCs-CM) IMPROVE CLINICAL OUTCOME WITH DELAYED ON Na-K ATPase CORNEAL ENDOTHELIAL PUMP RECOVERY IN PHACOEMULSIFIED RABBIT EYES.

Widayanto, Eko; Nurwasis; Hermawan, Dicky; Fidianto, Arief; Putri, Paramita; Rindiastuti, Yuyun; Sandhika, Willy; Ihsan, Igo Syaiful; Soebagdjo, Hendrian D.; Komaratih, Evelyn

To investigate the effect of LMSCs-CM on central corneal thickness (CCT) and Na /K ATPase of endothelial cells expression inphacoemulsifiedrabbit eyes. This was a true experimental laboratory study on rabbits using a pre and posttest control group design. Approximately 24 rabbit eyes were exposed to phacoemulsification ultrasound, then randomly divided into 2 groups. The control group was injected using 0.2 ml of physiological saline (BSS) intracamerally, while the treatment group was injected with 0.2 LMSCs-CM intracamerally. Central corneal thickness was evaluated on the 3rd day after treatment, followed by enucleation to analyzed Na /K ATPase expression using immunohistochemistry. The LMSCs-CM significantly reduced corneal edema and inflammation resulting in clinical improvement. There was no mean difference of CCT between groups (p = 0.372, α>0.05). However, CCT was higher in the control group (62.00 ± 27.41) when compared to the treatment group (50.00 ± 36.45 um). Similarly, the expression of Na / K ATPase in both groups was not significantly different (p = 0.973, α>0.05). After 3 days, LMSCs-CM exerted clinical improvements, but had a delay in functional recovery, in regards to Na /K ATPase expression in the corneal endothelial cells.

MESENCHYMAL stem cells; ENDOTHELIAL cells; PHACOEMULSIFICATION; IMMUNOHISTOCHEMISTRY; CONTROL groups

Biochemical & Cellular Archives, 2019, Vol 19, p4889

0972-5075

Academic Journal

10.35124/bca.2019.19.S2.4889