Hydroxylated-Benz[a]anthracenes Induce Two Apoptosis-Related Gene Expressions in the Liver of the Nibbler Fish Girella punctata.

Rafiuddin, Muhammad Ahya; Matsubara, Hajime; Hatano, Kaito; Honda, Masato; Toyota, Kenji; Kuroda, Kouhei; Tsunoda, Keito; Furusawa, Yukihiro; Tabuchi, Yoshiaki; Hirano, Tetsushi; Sakatoku, Akihiro; Hong, Chun-Sang; Srivastav, Ajai K.; Amornsakun, Thumronk; Shimizu, Nobuaki; Zanaty, Mohamed I.; Harumi, Tatsuo; Yamauchi, Kohei; Müller, Tamás; Tang, Ning

Polycyclic aromatic hydrocarbons (PAHs) are known to have toxic effects on fish. In this study, we examined the effects of benz[a]anthracene (BaA), a type of PAH, on fish liver metabolism. Nibbler fish (Girella punctata) were intraperitoneally injected with BaA (10 ng/g body weight) four times over a 10-day period. BaA significantly decreased known bone metabolism-related plasma factors such as calcium and inorganic phosphorus. Moreover, significant reductions were observed in the plasma levels of known liver metabolism-related factors, including ferrous ions, total bile acids, total bilirubin, free bilirubin, aspartate aminotransferase, and alkaline phosphatase. Interestingly, mono-hydroxylated metabolites of BaA, such as 3 hydroxylbenz[a]anthracene (3-OHBaA), were detected in the bile of BaA-injected nibbler fish. This hydroxylated form of BaA was found in its free form, rather than conjugated with glucuronic acid or sulfuric acid. Due to the lack of whole-genome sequence data for the nibbler fish, two nibbler fish-specific apoptosis-related factors (TNF receptor superfamily member 1A: tnfrsf1a and TNF superfamily member 10: tnfsf10) were isolated by De novo RNA sequencing. In a liver tissue culture, 3-OHBaA (10−6 M) significantly upregulated the expression of tnfrsf1a and tnfsf10 in the liver. These results provide the first evidence that 3-OHBaA metabolites exhibit toxic effects on the liver in teleost.

POISONS; POLYCYCLIC aromatic hydrocarbons; WHOLE genome sequencing; IRON ions; TUMOR necrosis factor receptors

Toxics, 2024, Vol 12, Issue 12, p915

2305-6304

Academic Journal

10.3390/toxics12120915