Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot.

Gonçalves, Allan Cristian; Coelho, Aline Meireles; da Cruz Castro, Maria Laura; Pereira, Renata Rebeca; da Silva Araújo, Natalia Pereira; Ferreira, Flávia Monteiro; Machado Júnior, Pedro Alves; Pio, Sirlaine; Vital, Camilo Elber; Bezerra, Frank Silva; Talvani, André; de Castro Borges, William; de Oliveira, Emerson Cruz; Costa, Daniela Caldeira

Paracetamol (APAP) overdose is the leading cause of drug-induced liver injury, leading to acute liver failure. However, the role of concurrent acute or chronic ethanol ingestion in this context requires further clarification. In this study, we investigated the effects of acute and chronic ethanol ingestion on APAP-induced hepatotoxicity. Male C57BL/6 mice were randomly allocated into four groups: control (C; water 2×/day for 7 days); APAP (single dose of APAP, 500 mg/kg); acute ethanol (AE; a single ethanol dose—10 mL/kg, and one hour later an overdose of APAP—500 mg/kg); chronic ethanol (CE; ethanol—10 mL/kg, 2×/day for 7 days; and on the last day, an overdose of APAP—500 mg/kg). The results showed that AE induced heightened liver damage, increased necrotic area, and elevated levels of ALT, AST, TBARS, and oxidized glutathione compared to the control group. The AE group exhibited diminished glutathione availability and elevated CYP2E1 levels compared to the other groups. CE maintained a hepatic profile similar to that of the control group in terms of necrosis index, ALT and AST levels, GSH/GSSG ratio, and CYP2E1 activity, along with the upregulation of gene expression of the glucuronidation enzyme compared to the APAP group. Proteomic analysis revealed that the AE protein profile closely resembled that of the APAP group, whereas the C and CE groups were clustered together. In conclusion, ethanol consumption differentially modulated APAP overdose-induced liver damage. Acute consumption exacerbated hepatotoxicity, similar to an APAP overdose alone, whereas chronic consumption appeared to mitigate this injury, at least within the parameters assessed in this study.

LIVER failure; LABORATORY mice; CYTOCHROME P-450 CYP2E1; DRUG side effects; HEPATOTOXICOLOGY

Toxics, 2024, Vol 12, Issue 12, p857

2305-6304

Academic Journal

10.3390/toxics12120857