Modulatory Role of Autophagy in Metformin Therapeutic Activity toward Doxorubicin-Induced Nephrotoxicity.

Antar, Samar A.; Abd-Elsalam, Marwa; Abdo, Walied; Abdeen, Ahmed; Abdo, Mohamed; Fericean, Liana; Raslan, Nahed A.; Ibrahim, Samah F.; Sharif, Asmaa F.; Elalfy, Amira; Nasr, Hend E.; Zaid, Ahmed B.; Atia, Rania; Atwa, Ahmed M.; Gebba, Mohammed A.; Alzokaky, Amany A.

Doxorubicin (DOX) is a frequent chemotherapeutic drug used to treat various malignant tumors. One of the key factors that diminish its therapeutic importance is DOX-induced nephrotoxicity. The first-line oral antidiabetic drug is metformin (Met), which also has antioxidant properties. The purpose of our study was to investigate the underlying molecular mechanisms for the potential protective effects of Met on DOX-triggered nephrotoxicity. Four animal groups were assigned as follows; animals received vehicle (control group), 200 mg/kg Met (Met group), DOX 15 mg/kg DOX (DOX group), and a combination of DOX and Met (DOX/Met group). Our results demonstrated that DOX administration caused marked histological alterations of widespread inflammation and tubular degeneration. Notably, the DOX-induced dramatic up-regulation of the nuclear factor-kappa B/P65 (NF-κB/P65), microtubule-associated protein light chain 3B (LC3B), neutrophil gelatinase-associated lipocalin (NGAL), interleukin-1beta (IL-1β), 8-hydroxy-2′ -deoxyguanosine (8-OHdG), and Beclin-1 in renal tissue. A marked increase in the malondialdehyde (MDA) tissue level and a decrease in the total antioxidant capacity (TAC) were also recorded in DOX-exposed animals. Interestingly, Met could minimize all histopathological changes as well as the disruptions caused by DOX in the aforementioned measures. Thus, Met provided a workable method for suppressing the nephrotoxicity that occurred during the DOX regimen via the deactivation of the Beclin-1/LC3B pathway.

DOXORUBICIN; LIPOCALIN-2; NEPHROTOXICOLOGY; OXIDANT status; MICROTUBULE-associated proteins; METFORMIN

Toxics, 2023, Vol 11, Issue 3, p273

2305-6304

Academic Journal

10.3390/toxics11030273