The search for innovative solutions to reduce food losses and waste remains a priority in the supply chain, yet past research has largely overlooked comprehensive packaging designs that address both operational and environmental challenges. Persistent issues, such as inadequate standardization, poor mechanical resistance, and insufficient ventilation in conventional packaging, significantly contribute to food losses, particularly in transportation and storage stages. Within this scope, this study bridges these gaps by developing differentiated packaging designs tailored to mitigate food losses in the fruits and vegetables (FV) supply chain. Employing a qualitative case study approach, data collection involved interviews, document analysis, and field research at CEAGESP. The findings revealed critical deficiencies in current practices, including fragile materials and ineffective ventilation. In response, five innovative packaging prototypes were developed using Autodesk Inventor and SolidWorks, featuring enhanced mechanical strength, standardized dimensions, and strategic ventilation. These prototypes aim to preserve FV during transport and storage while improving logistical efficiency and sustainability. By presenting practical, eco-efficient packaging solutions, this study advances the field and provides a foundation for future research and industry implementation.