Terahertz Spectroscopic Signatures of Microcystin Aptamer Solution Probed with a Microfluidic Chip.

Zhang, Mingkun; Yang, Zhongbo; Tang, Mingjie; Wang, Deqiang; Wang, Huabin; Yan, Shihan; Wei, Dongshan; Cui, Hong-Liang

Terahertz signature detection of biological samples in aqueous solution remains a great challenge due to the strong terahertz absorption of water. Here we propose a new preparation process for fabricating a microfluidic chip and use it as an effective sensor to probe the terahertz absorption signatures of microcystin aptamer (a linear single-stranded DNA with 60 nucleotides) dissolved in TE buffer with different concentrations. The microfluidic chip made of silicon includes thousands of 2.4 μm × 2.4 μm square-cross-section channels. One repeatable terahertz absorption signature is detected and recognized around 830 GHz, fitted to a Lorentz oscillator. This signature is theorized to originate from the bending of hydrogen bonds formed between adjacent hydrated DNA bases surrounded by water molecules. Furthermore, the low-lying vibrational modes are also investigated by molecular dynamics simulations which suggest that strong resonant oscillations are highly probable in the 815–830 GHz frequency band.

TERAHERTZ spectroscopy; MICROCYSTINS; APTAMERS; AQUEOUS solutions; MICROFLUIDIC devices

Sensors (14248220), 2019, Vol 19, Issue 3, p534

1424-8220

Academic Journal

10.3390/s19030534