We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and Puncture.
- Authors
Kim, Ok-Hyeon; Park, Jun-Hyung; Son, Jong-In; Yoon, Ok-Ja; Lee, Hyun-Jung; Heo, June-seok
- Abstract
Suitable scaffolds with appropriate mechanical and biological properties can improve mesenchymal stromal cell (MSC) therapy. Because silk fibroins (SFs) are biocompatible materials, they were electrospun and applied as scaffolds for MSC therapy. Consequently, interferon (IFN)-primed human bone marrow MSCs on SF nanofibers were administered into a polymicrobial sepsis murine model. The IL-6 level gradually decreased from 40 ng/mL at 6 h after sepsis to 35 ng/mL at 24 h after sepsis. The IL-6 level was significantly low as 5 ng/mL in primed MSCs on SF nanofibers, and 15 ng/mL in primed MSCs on the control surface. In contrast to the acute response, inflammation-related factors, including HO-1 and COX-2 in chronic liver tissue, were effectively inhibited by MSCs on both SF nanofibers and the control surface at the 5-day mark after sepsis. An in vitro study indicated that the anti-inflammatory function of MSCs on SF nanofibers was mediated through enhanced COX-2-PGE2 production, as indomethacin completely abrogated PGE2 production and decreased the survival rate of septic mice. Thus, SF nanofiber scaffolds potentiated the anti-inflammatory and immunomodulatory functions of MSCs, and were beneficial as a culture platform for the cell therapy of inflammatory disorders.
- Subjects
MESENCHYMAL stem cells; SEPSIS; TISSUE scaffolds; SILK fibroin; SURVIVAL rate; BIOMEDICAL materials
- Publication
Polymers (20734360), 2021, Vol 13, Issue 9, p1433
- ISSN
2073-4360
- Publication type
Academic Journal
- DOI
10.3390/polym13091433