Nutraceutical Capsules LL1 and Silymarin Supplementation Act on Mood and Sleep Quality Perception by Microbiota–Gut–Brain Axis: A Pilot Clinical Study.

Santamarina, Aline Boveto; Nehmi Filho, Victor; Freitas, Jéssica Alves de; Franco, Lucas Augusto Moysés; Fonseca, Joyce Vanessa; Martins, Roberta Cristina; Turri, José Antônio Orellana; Silva, Bruna Fernanda Rio Branco da; Gusmão, Arianne Fagotti; Olivieri, Eloísa Helena Ribeiro; Otoch, José Pinhata; Pessoa, Ana Flávia Marçal

Stress, unhealthy lifestyle, and sleep disturbance worsen cognitive function in mood disorders, prompting a rise in the development of integrative health approaches. The recent investigations in the gut–brain axis field highlight the strong interplay among microbiota, inflammation, and mental health. Thus, this study aimed to investigate a new nutraceutical formulation comprising prebiotics, minerals, and silymarin's impact on microbiota, inflammation, mood, and sleep quality. The study evaluated the LL1 silymarin capsule supplementation over 180 days in overweight adults. We analyzed the fecal gut microbiota using partial 16S rRNA sequences, measured cytokine expression via CBA, collected anthropometric data, quality of life, and sleep questionnaire responses, and obtained plasma samples for metabolic and hormonal analysis at baseline (T0) and 180 days (T180) post-supplementation. Our findings revealed significant reshaping in gut microbiota composition at the phylum, genus, and species levels, especially in the butyrate-producer bacteria post-supplementation. These changes in gut microbiota were linked to enhancements in sleep quality, mood perception, cytokine expression, and anthropometric measures which microbiota-derived short-chain fatty acids might enhance. The supplementation tested in this study seems to be able to improve microbiota composition, reflecting anthropometrics and inflammation, as well as sleep quality and mood improvement.

FECES; SHORT-chain fatty acids; RESEARCH funding; BRAIN; FLAVONOIDS; PREBIOTICS; PILOT projects; QUESTIONNAIRES; GUT microbiota; FUNCTIONAL foods; GASTROINTESTINAL system; RNA; BACTERIA; QUALITY of life; BLOOD plasma; AFFECT (Psychology); SLEEP quality; MINERALS; INFLAMMATION; CYTOKINES; ANTHROPOMETRY; BUTYRIC acid; PHARMACEUTICAL encapsulation; DIETARY supplements; OBESITY

Nutrients, 2024, Vol 16, Issue 18, p3049

2072-6643

Academic Journal

10.3390/nu16183049