Polymer–Colloid Complexes Based on Cationic Imidazolium Amphiphile, Polyacrylic Acid and DNA Decamer.

Kuznetsova, Darya A.; Gabdrakhmanov, Dinar R.; Kuznetsov, Denis M.; Lukashenko, Svetlana S.; Zakharov, Valery M.; Sapunova, Anastasiia S.; Amerhanova, Syumbelya K.; Lyubina, Anna P.; Voloshina, Alexandra D.; Salakhieva, Diana V.; Zakharova, Lucia Ya.

The solution behavior and physicochemical characteristics of polymer–colloid complexes based on cationic imidazolium amphiphile with a dodecyl tail (IA-12) and polyacrylic acid (PAA) or DNA decamer (oligonucleotide) were evaluated using tensiometry, conductometry, dynamic and electrophoretic light scattering and fluorescent spectroscopy and microscopy. It has been established that PAA addition to the surfactant system resulted in a ca. 200-fold decrease in the aggregation threshold of IA-12, with the hydrodynamic diameter of complexes ranging within 100–150 nm. Electrostatic forces are assumed to be the main driving force in the formation of IA-12/PAA complexes. Factors influencing the efficacy of the complexation of IA-12 with oligonucleotide were determined. The nonconventional mode of binding with the involvement of hydrophobic interactions and the intercalation mechanism is probably responsible for the IA-12/oligonucleotide complexation, and a minor contribution of electrostatic forces occurred. The latter was supported by zeta potential measurements and the gel electrophoresis technique, which demonstrated the low degree of charge neutralization of the complexes. Importantly, cellular uptake of the IA-12/oligonucleotide complex was confirmed by fluorescence microscopy and flow cytometry data on the example of M-HeLa cells. While single IA-12 samples exhibit roughly similar cytotoxicity, IA-12–oligonucleotide complexes show a selective effect toward M-HeLa cells (IC50 1.1 µM) compared to Chang liver cells (IC50 23.1 µM).

POLYACRYLIC acid; INTERCALATION reactions; LIVER cells; CATIONIC polymers; DNA; FLUORESCENCE microscopy; HYDROPHOBIC interactions; OLIGONUCLEOTIDES

Molecules, 2021, Vol 26, Issue 8, p2363

1420-3049

Academic Journal

10.3390/molecules26082363