Antibacterial Effects of Afzelin Isolated from Cornus macrophylla on Pseudomonas aeruginosa, A Leading Cause of Illness in Immunocompromised Individuals.

Sang Yeol Lee; Young-Jin So; Moon Sam Shin; Jae Youl Cho; Jongsung Lee

The crude ethyl acetate extract of the leaves of Cornus macrophylla showed antibacterial activity against Pseudomonas aeruginosa, a leading cause of illness in immunocompromised individuals. Bioactivity-guided separation led to the isolation of kaempferol 3-O-α-L-rhamnopyranoside (afzelin). The structure was determined based on evaluation of its spectroscopic (UV, MS, and NMR) data. The minimum inhibitory concentration (MIC) of afzelin against Pseudomonas aeruginosa was found to be 31 αg/mL. In addition, the results indicated that a hydroxyl group at C3 of the C-ring of the flavone skeleton and the rhamnose group may act as a negative factor and an enhancing factor, respectively, in the antibacterial activities of afzelin.

PSEUDOMONAS aeruginosa infections; DIAGNOSIS; ANTIBACTERIAL agents; COMMON witch hazel; NUCLEAR magnetic resonance; PREVENTION

Molecules, 2014, Vol 19, Issue 3, p3173

1420-3049

Academic Journal

10.3390/molecules19033173