Development of a Lab-on-a-Disk Platform with Digital Imaging for Identification and Counting of Parasite Eggs in Human and Animal Stool.

Sukas, Sertan; Van Dorst, Bieke; Kryj, Agata; Lagatie, Ole; De Malsche, Wim; Stuyver, Lieven J.

We present a lab-on-a-disk technology for fast identification and quantification of parasite eggs in stool. We introduce a separation and packing method of eggs contained in 1 g of stool, allowing for removal of commonly present solid particles, fat droplets and air bubbles. The separation is based on a combined gravitational and centrifugal flotation, with the eggs guided to a packed monolayer, enabling quantitation and identification of subtypes of the eggs present in a single field of view (FOV). The prototype was tested with stool samples from pigs and humans infected with intestinal parasites (soil-transmitted helminths eggs). The quality of the images created by this platform was appropriate for identification and quantification of egg types present in the sample.

EGGS; DIGITAL image processing; INTESTINAL parasites; BIRD eggs; IDENTIFICATION; PARASITES; BROOD parasitism; CELL separation

Micromachines, 2019, Vol 10, Issue 12, p852

2072-666X

Academic Journal

10.3390/mi10120852