Role Assessment of Water-Soluble Pharmaceutical Form of Phosphatidylcholine on the Catalytic Activity of Cytochrome P450 2C9 and 2D6.

Koroleva, Polina I.; Bulko, Tatiana V.; Kuzikov, Alexey V.; Gilep, Andrei A.; Romashova, Yulia A.; Tichonova, Elena G.; Kostrukova, Lyubov V.; Archakov, Alexander I.; Shumyantseva, Victoria V.

This study aimed to investigate whether the water-soluble pharmaceutical form of phosphatidylcholine nanoparticles (wPC) stimulated the catalytic activity of CYP enzymes 2C9 and 2D6. We have shown that electroenzymatic CYP2C9 catalysis to nonsteroidal anti-inflammatory drug naproxen as a substrate was enhanced from 100% to 155% in the presence of wPC in media. Electroenzymatic CYP2D6 activity in the presence of the adrenoceptor-blocking agent bisoprolol as a substrate was elevated significantly from 100% to 144% when wPC was added to potassium phosphate buffer solution. These results indicate the ability of wPC in the form of the phospholipid ultra-small nanoparticles to work as a membrane additive and crowding agent to accelerate the electroenzymatic reactions of cytochrome P450.

CYTOCHROME P-450; ELECTROCHEMICAL analysis; CATALYTIC activity; CYTOCHROME P-450 CYP2D6; POTASSIUM phosphates

International Journal of Molecular Sciences, 2025, Vol 26, Issue 1, p4

1661-6596

Academic Journal

10.3390/ijms26010004