Electrochemical Immunosensors on Laser-Induced Graphene Platforms for Monitoring of Anti-RBD Antibodies After SARS-CoV-2 Infection.

Martins, Beatriz R.; Andrade, Cristhianne Molinero R.; Simão, Guilherme F.; de Paula Martins, Rhéltheer; de Faria, Lucas V.; Matias, Tiago A.; Júnior, Virmondes Rodrigues; Munoz, Rodrigo Alejandro Abarza; Alves, Renata Pereira

The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has posed a major challenge to global health. The development of fast, accurate, and accessible diagnostic methods is essential in controlling the disease and mitigating its impacts. In this context, electrochemical biosensors present themselves as promising tools for the efficient monitoring of SARS-CoV-2 infection. We have developed a highly specific biosensor for the detection of anti-SARS-CoV-2 antibodies in patient sera. The use of the RBD-S region as an antigen, although purified to minimize cross-linking, poses a specific challenge. The structural similarity between SARS-CoV-2 and other respiratory viruses, as well as the complexity of the serum matrix, hinders robust analytical strategies to ensure diagnostic accuracy. This work presents a novel immunosensor for COVID-19 diagnosis using laser-induced graphene (LIG) electrodes subjected to electrochemical reduction with graphene (named rGraphene-LIG). In the present study, we chose an initial approach focused on demonstrating the concept and evaluating the feasibility of the rGraphene-LIG sensor for SARS-CoV-2 detection. The rGraphene-LIG electrodes presented a notable current increase for the redox probe in the aqueous solution of a mixture of 5 mmol L−1 potassium ferricyanide/ferrocyanide ([Fe(CN)6]3−/[Fe(CN)6]4−) in 0.1 mol L−1 KCl (pH set at 7.4). As a proof of concept, the rGraphene-LIG electrode was applied for antibody determination in real samples using cyclic voltammetry, and a limit of detection (LOD) of 0.032 μg L−1 was achieved. When determining antigens in commercial samples, we obtained an LOD of 560 ηg mL−1 and a limit of quantification of 1677 ηg mL−1. The results of the electrochemical experiments were in accordance with the surface roughness obtained from atomic force microscopy images. Based on these results, the rGraphene-LIG electrode is shown to be an excellent platform for immunoglobulin detection when present in individuals after antigenic exposure caused by SARS-CoV-2.

ATOMIC force microscopy; SARS-CoV-2; ELECTROCHEMICAL electrodes; ELECTROCHEMICAL experiments; CYCLIC voltammetry

Biosensors (2079-6374), 2024, Vol 14, Issue 11, p514

2079-6374

Academic Journal

10.3390/bios14110514