Results
Title

A Floating Capsule Electrochemical System for In Situ and Multichannel Ion-Selective Sensing.

Authors

Yang, Jie; Ding, Ao; Zhou, Jia-Le; Yan, Bing-Yong; Gu, Zhen; Wang, Hui-Feng

Abstract

Free-floating electrochemical sensors are promising for in situ bioprocess monitoring with the advantages of movability, a lowered risk of contamination, and a simplified structure of the bioreactor. Although floating sensors were developed for the measurement of physical and chemical indicators such as temperature, velocity of flow, pH, and dissolved oxygen, it is the lack of available electrochemical sensors for the determination of the inorganic ions in bioreactors that has a significant influence on cell culture. In this study, a capsule-shaped electrochemical system (iCapsuleEC) is developed to monitor ions including K , NH4 , Na , Ca2 , and Mg2 based on solid-contact ion-selective electrodes (SC-ISEs). It consists of a disposable electrochemical sensor and signal-processing device with features including multichannel measurement, self-calibration, and wireless data transmission. The capacities of the iCapsuleEC were demonstrated not only for in situ measurement of ion concentrations but also for the optimization of the sensing electrodes. We also explored the possibility of the system for use in detection in simulated cell culture media.

Subjects

OXYGEN detectors; ELECTROCHEMICAL sensors; BIOTECHNOLOGICAL process monitoring; CALCIUM ions; CELL culture; PHYSICAL measurements; INDICATORS & test-papers; FLOW velocity

Publication

Biosensors (2079-6374), 2023, Vol 13, Issue 10, p914

ISSN

2079-6374

Publication type

Academic Journal

DOI

10.3390/bios13100914