Results
Title

The nucleolin MoNsr1 plays pleiotropic roles in the pathogenicity and stress adaptation in the rice blast fungus Magnaporthe oryzae.

Authors

Zhen Zhang; Islam, Mohammad Shafiqul; Jiuzhi Xia; Xiangyang Feng; Noman, Muhammad; Jing Wang; Zhongna Hao; Haiping Qiu; Rongyao Chai; Yingying Cai; Yanli Wang; Jiaoyu Wang

Abstract

The rice blast disease, caused by the fungus Magnaporthe oryzae, is a significant agricultural problem that adversely impacts rice production and food security. Understanding the precise molecular pathways involved in the interaction between the pathogen and its host is crucial for developing effective disease management strategies. This study examines the crucial function of the nucleolin MoNsr1 in regulating M. oryzae physiological functions. DMoNsr1 deletion mutants showed reduced fungal growth, asexual sporulation, and pathogenicity compared to the wild-type. Mutants exhibited impaired conidial germination and appressoria formation, reducing infection progression. Additionally, DMoNsr1 deletion mutant had less turgor pressure, confirming that MoNsr1 is essential for cell wall biogenesis and resistant to external stresses. Furthermore, DMoNsr1 deletion mutant showed enhanced sensitivity to oxidative stress, reactive oxygen species, and cold tolerance. Our results offer a thorough understanding of the function of MoNsr1 in the virulence and stressresilient capability in M. oryzae. These findings provide insights into the novel targets and contribute to the emergence of innovative approaches for managing rice blast disease.

Subjects

PYRICULARIA oryzae; REACTIVE oxygen species; AGRICULTURE; NUCLEOLIN; DISEASE management; RICE blast disease

Publication

Frontiers in Plant Science, 2024, p1

ISSN

1664-462X

Publication type

Academic Journal

DOI

10.3389/fpls.2024.1482934