Results
Title

Retrospect and prospect of Nicotiana tabacum genome sequencing.

Authors

Zhijun Tong; Yujie Huang; Qian-Hao Zhu; Longjiang Fan; Bingguang Xiao; Enhui Shen

Abstract

Investigating plant genomes offers crucial foundational resources for exploring various aspects of plant biology and applications, such as functional genomics and breeding practices. With the development in sequencing and assembly technology, several Nicotiana tabacum genomes have been published. In this paper, we reviewed the progress on N. tabacum genome assembly and quality, from the initial draft genomes to the recent high-quality chromosome-level assemblies. The application of long-read sequencing, optical mapping, and Hi-C technologies has significantly improved the contiguity and completeness of N. tabacum genome assemblies, with the latest assemblies having a contig N50 size over 50 Mb. Despite these advancements, further improvements are still required and possible, particularly on the development of pan-genome and telomere-totelomere (T2T) genomes. These new genomes will capture the genomic diversity and variations among different N. tabacum cultivars and species, and provide a comprehensive view of the N. tabacum genome structure and gene content, so to deepen our understanding of the N. tabacum genome and facilitate precise breeding and functional genomics.

Subjects

PLANT genomes; PAN-genome; FUNCTIONAL genomics; TOBACCO; GENOMES

Publication

Frontiers in Plant Science, 2024, p1

ISSN

1664-462X

Publication type

Academic Journal

DOI

10.3389/fpls.2024.1474658