The aim of this study is to compare the vertical jump, squat strength output, and sprint times of young football players playing in different levels and leagues. A total of 44 players voluntarily participated in the study, including 22 players from the TFF U-17 National Team and 22 players from Erzurumspor FK. Participants were subjected to body composition assessment, vertical jump test, squat test, and 30-meter sprint tests. After assessing the normality distribution of the measured parameters using Skewness, Kurtosis, and Shapiro-Wilk tests, it was determined that the data exhibited a normal distribution (±2 standard deviations). As the data exhibited a normal distribution, Independent Samples t-test was applied to determine differences between the leagues. In this study, a significance level of p<0.05 was adopted. When comparing the values of the groups, it was observed that there was a significant difference in hang time, jump height, and sprint times in favor of the national team players (p<0.05), while there was no statistically significant difference in anaerobic power values. It was found that national team players competing at the international level had a higher level of physical capacity compared to players competing at the national level. Additionally, through an investigation of different physical and physiological test results, differences can be identified, and the training content for players can be updated to reduce the gap.