Hypoglycemic, hepatoprotective and molecular docking studies of 5-[(4-chlorophenoxy) methyl]-1,3,4-oxadiazole-2-thiol.

Shehzadi, Naureen; Hussain, Khalid; Bukhari, Nadeem Irfan; Islam, Muhammad; Khan, Muhammad Tanveer; Salman, Muhammad; Siddiqui, Sabahat Zahra; Aziz-Ur-Rehman; Abbasi, Muhammad Athar

The present study aimed at the evaluation of anti-hyperglycemic and hepatoprotective potential of a new drug candidate, 5-[(4-chlorophenoxy) methyl]-1,3,4-oxadiazole-2-thiol (OXCPM) through in vitro and in vivo assays, respectively. The compound displayed excellent dose-dependent α-amylase (28.0-92.0%), α-glucosidase (40.3-93.1%) and hemoglobin glycosylation (9.0%- 54.9%) inhibitory effects and promoted the uptake of glucose by the yeast cells (0.2 to 26.3%). The treatment of the isoniazid- and rifampicin- (p.o., 50 mg/kg of each) intoxicated rats with OXCPM (100 mg/kg, p.o.) resulted in restoring the normal serum levels of the non-enzymatic (total bilirubin, total protein and albumin) and bringing about a remarkable decrease in the levels of enzymatic (alanine transaminases, aspartate transaminases and alkaline phosphatase) biomarkers. The molecular docking studies indicated high binding affinity of the compound for hyperglycemia-related protein targets; fructose-1,6-bisphosphatase, beta2-adrenergic receptors and glucokinase. The results indicate that OXCPM may not only reduce hyperglycemia by enzyme inhibition but also the disease complications through protection of hemoglobin glycosylation and hepatic injury.

HYPOGLYCEMIC agents; HEMOGLOBINS; GLYCOSYLATION; GLUCOSE; ENZYMES

Bangladesh Journal of Pharmacology, 2018, Vol 13, Issue 2, p149

1991-007X

Academic Journal

10.3329/bjp.v13i2.35514