Title: Use of Magnetic Nanoparticles Fe<sub>3</sub>O<sub>4</sub> in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase.
Authors: Grebennikova, Olga V.; Sulman, Aleksandrina M.; Matveeva, Valentina G.
Abstract: In this study, magnetic nanoparticles Fe3O4 synthesized by the polyol method were used to immobilize Horseradish root peroxidase. The surface of magnetic nanoparticles was pretreated with tetra-ethyl orthosilicate, 3-aminopropyltriethoxysilane, and glutaraldehyde. As a result, two biocatalysts were obtained Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP. The activity of the synthesized biocatalysts was estimated spectrophotometrically in the oxidation reaction of 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) with hydrogen peroxide. The optimal conditions for this reaction were selected (pH 6.0, temperature 45 °C). It has been determined that biocatalysts Fe3O4/APTS/GA/HRP and Fe3O4/TEOS/APTS/GA/HRP slightly decreased their activity during 5 consecutive experiments, by 19 and 15 %.
Subjects: MAGNETIC nanoparticles; HORSERADISH; PEROXIDASE; GLUTARALDEHYDE; HYDROGEN peroxide
Publication: CET Journal - Chemical Engineering Transactions, 2022, Vol 94, p673
ISSN: 1974-9791
Publication type: Academic Journal
DOI: 10.3303/CET2294112

Use of Magnetic Nanoparticles Fe<sub>3</sub>O<sub>4</sub> in the Synthesis of Biocatalysts Based on Horseradish Root Peroxidase.

Grebennikova, Olga V.; Sulman, Aleksandrina M.; Matveeva, Valentina G.