Objective: the objective of this work was to assess the effect of bull breed on sperm quality after thawing semen. Design/methodology/approach: ten plastic straws of 0.5 mL of frozen semen from three different breeds of bull were used, which were purchased from a commercial company. The bull breeds used were Charolais, Brahman and Simbrah. The straws were thawed at 37 °C for 40 s. Immediately afterwards motility, viability and acrosmosomal integrity (NAR) were assessed. Results: the results were for motility 75.0, 87.5 and 85%; for viability 74.5, 74.5 and 72.5%; and for NAR 97.1, 96.9 and 96.9% for the Charolais, Brahaman and Simbrah races, respectively. Limitations/implications: the straws used were obtained from a commercial company dedicated to the sale of frozen bovine semen. Findings/conclusions: the three bull breeds showed good sperm quality after thawing, which can be recommended to be used in artificial insemination without any problem.