BIOLOGICAL ACTIVITY, CYTOTOXICITY, AND PHYTOCHEMICAL CONTENT OF LEAF EXTRACTS OF MATOA (POMETIA PINNATA) AND PULASAN (NEPHELIUM RAMBOUTAN-AKE) FROM THE SAPINDACEAE FAMILY.

VIVIAN CHUA; LI JING CHONG; YAN TAN; XIN YING TENG; PUI EE NG; FATHMATH EMAN MOHAMED; CAI PING KOH; NAM WENG SIT

This study sought to assess leaf extracts of matoa (Pometia pinnata) and pulasan (Nephelium ramboutan-ake) for antibacterial, antifungal, antioxidant, and cytotoxic activities, beside phytochemical content. Four extracts (hexane, ethyl acetate, ethanol, and water) were obtained from each plant via sequential extraction. The antimicrobial activities were evaluated using colorimetric broth microdilution methods. The antioxidant activities were studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric-reducing antioxidant power (FRAP) assays. Cytotoxicity was examined using mouse fibroblasts NIH/3T3, while the phytochemical content was detected using various chemical tests. Among the extracts, only the water extract of matoa and ethyl acetate extract of pulasan exerted bactericidal effects on Bacillus cereus (minimum bactericidal concentration (MBC): 0.63 mg/mL) and Staphylococcus aureus (MBC: 1.25 mg/mL), respectively. In contrast, all extracts, except hexane extract, of both plants exhibited fungicidal effects with minimum fungicidal concentrations of 0.31-1.25 mg/mL. All extracts of matoa leaves displayed higher DPPH radical scavenging activity and FRAP than the extracts of pulasan leaves. Notably, the water extract of matoa showed the lowest half-maximal inhibitory concentration (IC50) value of 9.88 ± 1.70 µg/mL and the highest FRAP value of 6.11 ± 0.51 mmol Fe2 equivalent/g extract (n=3). These antioxidant activities were significantly correlated (P < 0.01) with the amounts of phenolic compounds in the extracts. All extracts of both plants, except the water extracts, showed significant toxicities (P < 0.05) towards NIH/3T3 cells. Alkaloids, terpenoids, saponins, and glycosides were detected in the leaves of both plants. The matoa and pulasan leaves contained bioactive compounds with antibacterial, antifungal, and antioxidant activities.

NATIONAL Institutes of Health (U.S.); CHEMICAL testing; ETHYL acetate; BACILLUS cereus; CYTOTOXINS; PHENOLS

Acta Poloniae Pharmaceutica, 2024, Vol 81, Issue 3, p467

0001-6837

Academic Journal

10.32383/appdr/192036