OUTCOME OF CO-ADMINISTRATION OF SEA BUCKTHORN FRUIT IN HIGH FAT DIET WISTAR RATS.

TIMNEA, OLIVIA; VIRGOLICI, BOGDANA; GAGNIUC, ELVIRA; MOHORA, MARIA; MIRICESCU, DANIELA; GRADINARU, DANIELA; STANESCU-SPINU, IULIA; DOBRE, MARIA

Obesity is a risk factor for diabetes, cardiovascular diseases, cognitive impairment and sarcopenia. Sea Buckthorn fruit may have beneficial effects on muscle mass and memory. This study evaluated the metabolic impact of a high-fat diet on young Wistar rats and the effects of co-administering Sea Buckthorn fruit on skeletal muscle and brain tissues. Rats aged 10 weeks were divided into three groups (n = 6 per group) and for the next 8 weeks were raised on: standard diet (Control group), highfat diet (HFD group) and high-fat diet with Sea Buckthorn fruit (0.3 g fruit/100g rat weight/day), (HFDSB group). In the HFD group, the gastrocnemius muscle showed decreased phosphorylation of Akt protein (pAkt 5.77%) and the haematoxylin-eosin staining demonstrated brain and muscle pathological changes. Serum tests indicated metabolic imbalances. The coadministration of the Buckthorn fruit to the high fat diet reduced the intervisceral fat, glycaemia (p < 0.05), triglyceridemia (p < 0.05) and resistininemia (p < 0.05). It also increased the insulin sensitivity (pAkt 21.2%, p < 0.05) and the tissue histological appearance for the brain and for the muscle was almost normal. In conclusion, Sea Buckthorn fruit mitigated skeletal muscle and brain damage and enhanced insulin sensitivity in rats on a high-fat diet.

HIGH-fat diet; SEA buckthorn; LABORATORY rats; EPICATECHIN; INSULIN sensitivity; REDUCING diets; ELLAGIC acid

Farmacia, 2024, Vol 72, Issue 3, p581

0014-8237

Academic Journal

10.31925/farmacia.2024.3.12