The anti-migratory activity of a new curcumin analog, CCA-1.1, against T47D breast cancer cells.

WULANDARI, FEBRI; UTOMO, ROHMAD YUDI; NOVITASARI, DHANIA; IKAWATI, MUTHI'; KIRIHATA, MITSUNORI; KATO, JUN-YA; MEIYANTO, EDY

Chemoprevention-Curcumin Analog 1.1 (CCA-1.1), a novel curcumin analog, has been proven to have antiproliferative effects against T47D cells covering the cytotoxic activity in correlation with cell cycle inhibition, apoptosis induction, ROS generation, and senescence induction. This study investigated the antimigratory effect of CCA-1.1 on T47D cells. The expressions status of MMP-2 and MMP-9 in human breast cancers were obtained through GEPIA online databases. The cytotoxic evaluation was conducted by MTT assay. Then, cell migration and MMPs expression after CCA-1.1 treatment on T47D cells were measured using scratch wound healing and gelatin zymography assays, respectively. Moreover, molecular docking analysis was used to assess the binding activity of CCA-1.1 to MMP-2 and MMP-9. Based on the TCGA dataset through GEPIA online database, MMP-9 expressions were significantly higher in breast cancer tissues than normal breast tissues. We found that CCA-1.1 possesses cytotoxic effects similar to those of PGV-1 on T47D cells. CCA-1.1 also inhibited the migration of T47D cells and suppressed these MMP-2 and MMP-9 expressions. Furthermore, we found that CCA-1.1 possessed a higher binding affinity than PGV-1 to MMP-2 and MMP-9 proteins. These results confirmed that CCA-1.1 has potency as an anti-migratory agent and may apply for antimetastatic therapy.

BREAST cancer; CANCER cells; CURCUMIN; ONLINE databases; MATRIX metalloproteinases; CELL migration; BREAST

International Journal of Pharmaceutical Research (09752366), 2021, Vol 13, Issue 1, p2877

0975-2366

Academic Journal

10.31838/ijpr/2021.13.01.421