Antimicrobial and Cytotoxic Activities of A compound Produced by An Endophytic Fungus Isolated from The Leaves of Coleus amboinicus Lour.

ASTUTI, PUJI; ROLLANDO, ROLLANDO; PRATOKO, DWI KOKO; WAHYUONO, SUBAGUS; NURROCHMAD, ARIEF

Various compounds are produced by endophytes and possess antimicrobial activities. An endophytic fungus originated from Coleus amboinicus was discovered to produce compounds having antimicrobial activities. This study was intended to explore the bioactive compounds responsible for antibacterial activities and possibly also for their potential as anticancer agents. Isolation of the bioactive compounds was conducted by bioassayguided fractionation using disc diffusion method for antimicrobial screening activities. The antimicrobial potential was determined by IC50 and MBC (Minimum Bactericidal Concentration) values. Cytotoxicity testing was conducted by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against T47D breast cancer cell line and normal Vero cells. Structure elucidation of the bioactive compound was done by mean of spectroscopic data. Disc diffusion test of four fractions followed by examination of IC50 and MBC values of isolated compounds showed that a compound within fraction 2 was found to be the most active one with IC50 of 0.73,1.24,1.17, 0.31 and 1.27 µg/ml against S. aureus, B. subtilis, E. coli, S. mutans, and P. aeruginosa respectively. This compound also killed T47D (IC50 of 174 µg/ml) and Vero cells (IC50 of 214 µg/ml). The bioactive compound was suggested to be an aromatic compound having methoxy, hydroxyl and methyl groups. These data demonstrated the potential of an endophytic fungus Athelia rolfsii as a producer of antimicrobial agents, yet its potential for producing anticancer agents still need to be explored.

ANTI-infective agents; ENDOPHYTIC fungi; ESCHERICHIA coli; BIOACTIVE compounds; ANTINEOPLASTIC agents; ANTIBACTERIAL agents

International Journal of Pharmaceutical Research (09752366), 2021, Vol 13, Issue 1, p2632

0975-2366

Academic Journal

10.31838/ijpr/2021.13.01.394