THE ANTICANCER EFFICACY OF INDONESIAN NUTMEG HYDROSOL EVALUATED THROUGH IN SILICO AND IN VITRO APPROACHES ON MCF7 AND 3T3 CELLS.

Widiyarti, G.; Sundowo, A.; Ernawati, T.; Filailla, E.; Artanti, N.; Ajie, F. T.; Subiyanto, B.; Laksmono, J. A.

Traditionally, the nutmeg plant (Myristica fragrans Hooutt) has been used to treat a variety of ailments in Indonesia. This study aimed to determine the anticancer efficacy of nutmeg hydrosol using in silico and in vitro methods. Nutmeg hydrosol refers to distilled water produced during the refining process of nutmeg oil, containing dissolved essential components. The LCMS/MS analysis result showed that the main components of hydrosol were Biondinin A, Edultin, Methyl-3-hydroxy-4-methoxybenzoate, Pluviatilol, Aviprin, Latifolone, and 2,4-Dihydroxyaceto-phenone. Based on the in silico analysis using the Autodock 4.2 method on 4WZV protein, the compounds had high binding energy, suggesting great potential as anticancers. Free radicals were shown to be actively inhibited by the hydrosol's polyphenols. Hydrosol was also classified as a very active antioxidant by the DPPH assay, which yielded an IC50 value of 45.3 µg/ml. Using the MTT method, an in vitro cytotoxicity investigation revealed that the substance was cytotoxic to MCF7 cells but harmless to normal cells (3T3), with IC50 values of 79.45 μg/ml and >1000 μg/ml, respectively. These findings indicated a favorable association between antioxidants and anticancer characteristics, indicating the possibility of using nutmeg hydrosol as a breast cancer anticancer drug.

NUTMEG tree; CYTOTOXINS; BINDING energy; ANTINEOPLASTIC agents; FREE radicals

Rasayan Journal of Chemistry, 2024, Vol 17, Issue 4, p1479

0974-1496

Academic Journal

10.31788/RJC.2024.1748890